PROTEIN SYNTHESIS IN SYNAPTOSOMAL FRACTIONS
نویسندگان
چکیده
منابع مشابه
Protein Synthesis in Synaptosomal Fractions
A quantitative ultrastructural radioautographic study of in vitro protein synthesis has been carried out in rat synaptosomal fractions incubated with tritiated leucine or a tritiated amino acid mixture. Analysis of grain density distribution demonstrated that presynaptic endings are labeled. 30-50% of the developed grains, representing tritiated amino acids incorporated into proteins, were rela...
متن کاملPROTEIN SYNTHESIS IN SYNAPTOSOMAL FRACTIONS Ultrastructural Radioautographic Study
A quantitative ultrastructural radioautographic study of in vitro protein synthesis has been carried out in rat synaptosomal fractions incubated with tritiated leucine or a tritiated amino acid mixture. Analysis of grain density distribution demonstrated that presynaptic endings are labeled. 30-50% of the developed grains, representing tritiated amino acids incorporated into proteins, were rela...
متن کاملEvidence for protein synthesis in synaptosomal membranes.
Evidence is presented that a brain fraction containing synaptosomal membrane fragments and ghosts prepared from lysed rat forebrain synaptosomes synthesize protein in a cell free system. The protein synthesis was dependent upon the addition of a synaptosomal membrane fraction, Mg++, monovalent cations, ATP, GTP, and enzyme factors prepared from intact synaptosomes. Protein synthesis was inhibit...
متن کاملEvidence for Protein Synthesis in Synaptosomal Membranes*
Evidence is presented that a brain fraction containing synaptosomal membrane fragments and ghosts prepared from lysed rat forebrain synaptosomes synthesize protein in a cell free system. The protein synthesis was dependent upon the addition of a synaptosomal membrane fraction, Mg++, monovalent cations, ATP, GTP, and enzyme factors prepared from intact synaptosomes. Protein synthesis was inhibit...
متن کاملProtein asymmetry in chick synaptosomal plasma membrane.
The topographical arrangement of chick synaptosomal plasma membrane polypeptides was examined by lactoperoxidase-catalyzed iodination and trypsin digestion of proteins exposed at the exterior surface. The ““I-labeling profile on sucrose gradients of lysed, subfractionated synaptosomes generally coincided with the activity of the membrane marker (Na+-K+)-activated ATPase, which suggested that la...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.52.3.526